Yuning Liu, Yuran Xue

(School of Life Sciences, Zhengzhou University)

Abstract：

Objective To explore the method of topical application of 1-chloro-2.4-dinitrobenzene (DNCB) for skin sensitization and stimulation of Kunming mouse model of atopic dermatitis. Method Ten Kunming mice were randomly divided into two groups (n=5). The model group mice were sensitized with 1% DNCB and stimulated with 4% SDS+0.5% DNCB, while the control group mice were sensitized with a mixture of acetone and olive oil solution and stimulated. The drug was applied to both ears and back skin. Observe the thickness of mouse ears, histopathological and immunological indicators, and evaluate the degree of dermatitis damage. Result topical application of DNCB can cause significant atopic dermatitis like skin lesions in Kunming mice. The model group mice showed dry skin, erythema, edema, and erosion. Histopathological analysis using HE staining showed thickening of the epidermis and dermis, excessive keratinization, and infiltration of inflammatory cells. Conclusion Repeated stimulation of Kunming mice with 1% DNCB, 4% SDS, and 0.5% DNCB can cause physiological and pathological changes in the skin, which are consistent with the clinical manifestations of AD patients. A model of atopic dermatitis has been successfully established. It can serve as an effective animal model for studying the etiology and treatment of Alzheimer's disease.

Key Words：

atopic dermatitis; kunming rat DNCB; model