Xuefei Zhang, Zhenqi Li

(College of Chemical and Pharmaceutical Engineering, Henan University of Science and Technology)

Abstract：

The biocatalytic conversion and fermentation technology of food and feed has become an important field to ensure table safety and green development. Its enzyme usage accounts for the largest proportion and fastest growth in the industrial enzyme preparation market. Enzyme preparations with excellent performance can significantly shorten the fermentation cycle, reduce production costs, improve the conversion rate of target products, and thereby enhance market competitiveness. In addition to using separated and extracted enzyme preparations, many fermentation processes in the food and feed processing industry still rely on microorganisms as cell factories for various enzyme catalyzed transformations. The fermentation process usually involves complex metabolic networks and regulatory mechanisms, and many systems cannot be genetically engineered, so rational genetic engineering modification is often difficult to meet application needs. Mutation breeding is still the main means of enzyme and microbial strain modification for food and feed processing, and new technologies need to be constantly developed to keep up with the times. The atmospheric pressure room temperature plasma (ARTP) mutagenesis breeding technology developed in recent years has been widely used in microbial breeding for food and feed processing due to its characteristics of simple operation, safety, environmental friendliness, high mutation rate, and large mutation storage capacity, and has achieved good results. Focus on the recent application progress of ARTP in food and feed processing enzyme preparations and fermentation microbial modification.

Key Words：

atmospheric pressure room temperature plasma (ARTP); enzyme preparations; food and feed processing; microbial fermentation; mutation breeding