Association between MSTN1 Gene Polymorphism and Growth Traits in Esox lucius

Huang Lianjie, Guo Li, Lou Minghui, Zhu Xiangwei, Wang Jia^*

Xinjiang Key Laboratory for Ecological Adaptation and Evolution of Extreme Environment Organisms / College of Life Sciences, Xinjiang Agricultural University Key

Abstract：

Esox lucius, commonly known as "Qiaoertai", is characterized by rich nutrition, delicious taste and rapid growth. As an important indigenous fish in the Xinjiang, E. lucius is a popular edible fish with good economic prospects. Myostatin (MSTN) is an essential protein for the normal operation of animal bodies and a negative regulator of muscle development. It plays an important role in muscle development and homeostasis maintenance, determining the overall muscle mass of animals. Single nucleotide polymorphism (SNP) marker loci associated with growth traits have been reported in many fish, but there are few reports on E. lucius. In this study, E. lucius was used as the research object, primers were designed to clone its MSTN1, and PCR product direct sequencing and Chromas software were used for screening and genotyping of gene polymorphism to identify SNP molecular markers of MSTN1 in E. lucius. At the molecular genetics level, this study provides a new tool for the genetic breeding, aiming to promote the breeding process of excellent varieties of E. lucius with high growth performance.

Key Words：

Esox lucius; MSTN1; SNP; growth traits; molecular marker-assisted breeding