Jun Li, Zunyuan Wang

(College of Chemistry and Chemical Engineering, Lanzhou Jiaotong University)

Abstract：

Electrochemiluminescence (ECL), as one of the branches of electrochemical technology, is a simple, efficient, and sensitive analytical method in the field of analytical chemistry research. With its advantages of high stability, low background, and wide dynamic range, ECL has been widely used in clinical diagnosis, food safety, and environmental monitoring. With the vigorous development of life sciences, modern medicine has shifted from the cellular level to the molecular level, placing higher demands on the specificity and sensitivity of ECL biosensors. The strategy of nucleic acid signal amplification and DNA nanomachines can effectively improve sensor performance. This article comprehensively discusses the signal amplification technologies associated with nucleic acids in recent years, with a focus on the application research of nucleic acid isothermal signal amplification strategy in ECL biosensors.

Key Words：

electrochemiluminescence; biosensors; nucleic acid signal amplification strategy