Yulin Hu, Yang Liu

(College of Metallurgy and Chemical Engineering, Jiangxi University of Science and Technology)

Abstract：

Clinically relevant molecules (including small molecules, nucleic acids, and proteins) have many biological functions, including storing and transmitting genetic information, regulating biological activities, transporting small molecules, and catalyzing reactions, among others. In addition, they can also serve as biomarkers for the diagnosis of many diseases. Therefore, the precise detection of these small molecules is of great significance for the diagnosis and treatment of diseases. Biosensors can quantitatively analyze analytes through output signals, and are widely used due to their high speed, sensitivity, and selectivity. In recent years, with the rise of DNA nanotechnology, the use of DNA functional materials to construct biosensors has significant implications for improving their sensitivity and specificity. This paper combines signal amplification techniques such as target cyclic amplification, hybrid chain reaction, rolling ring amplification, and cascade catalytic amplification with DNA functional materials to enhance signal response and construct different novel sensitive biosensors.

Key Words：

signal amplification technology; DNA functional materials; biosensors