Tianling Liu, Jianmei Ma

(Xinjiang Academy of Agricultural Reclamation Sciences)

Abstract：

Virescent trait could be used as a natural marker for breeding to reduce time and process of hybrid breeding，moreover they were ideal mod⁃ el systems to study chlorophyll and chloroplast biosynthesis and biodegradation process.the virescent mutant in cotton is of great theoretical and practical implications. In this study，an F2 segregation population was constructed derived from 115-23（virscent mutant）and JSH1527（Normal green trait），based on the identification of virescent trait，30 virescent and 30 green plants were selected to construct a mixed pool，four samples（2 parent pools and 2 mixed pools）were sequenced，SNP-index were used for association analysis to determine the candidate regions of virescentgenes. As a result，840，612 SNPs were obtained between the two parents，one candidate physical regions showing confidence indices higher than 95% were obtained on chromosome D02，which contained 93 annotated genes，four of these genes were functional annotations related to chloroplast synthesis and may be candidate genes for the regulation of cotton leaf color. This study laid a foundation for further analyzing the genetic mechanism and clones of genes of cotton virescent formation.

Key Words：

Cotton; Virescent; BSA-Seq; Candidate genes